Argireline molecular structure
Argireline molecular structure
Approved
🧴Cosmetic & Topical

Argireline

Also known as: Acetyl Hexapeptide-8, Acetyl Hexapeptide-3

MW

888.90 Da

Formula

C34H60N14O12S

CAS

616204-22-9

Routes

1 route

Argireline (acetyl hexapeptide-3/acetyl hexapeptide-8) is a synthetic hexapeptide developed by Lipotec (now Lubrizol) as a topical alternative to botulinum toxin for reducing expression wrinkles. It works by inhibiting SNARE complex formation necessary for neurotransmitter release at the neuromuscular junction, reducing the muscle contractions that cause expression lines. Applied topically in cosmetic formulations at 5-10% concentrations, argireline has demonstrated wrinkle reduction of up to 30% in clinical studies without the injection requirement, onset delay, or safety risks of botulinum toxin. It is one of the most commercially successful cosmetic peptides, used in thousands of anti-wrinkle products worldwide.

Research Use Only— For educational and research purposes only

Research Applications

Anti-Wrinkle Cosmeceutical

Clinical studies show 17-30% wrinkle depth reduction after 30 days of topical application at 10% concentration. Most effective for periorbital (crow's feet) and forehead expression lines.

Botox Alternative Research

Studied as a non-invasive alternative to botulinum toxin injections for mild to moderate expression wrinkles.

Mechanism of Action

Argireline mimics the N-terminal end of SNAP-25, a key component of the SNARE complex that mediates synaptic vesicle fusion with the presynaptic membrane. By competing with endogenous SNAP-25, argireline destabilizes the SNARE complex (SNAP-25/syntaxin/VAMP), reducing acetylcholine vesicle exocytosis at neuromuscular junctions. This results in decreased muscle contraction intensity in facial expression muscles, softening dynamic wrinkles without complete paralysis.

Biological Pathways

SNARE complex (SNAP-25/syntaxin-1/VAMP-2) disruption. Reduced calcium-dependent vesicle fusion. Partial inhibition of neuromuscular transmission in superficial facial muscles.

Dosage Information

Typical dosage ranges for research applications. Always verify with current literature.
Frequency
Once or twice daily as serum/cream (5-10% concentration)
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Calculation Results

Concentration
2.5 mg/ml
Dose Volume
0.1 ml0.100 ml
Insulin Syringe
10 units
Doses per Vial
2020 doses @ 250 mcg

Syringe Fill Level (100u syringe)

05010010.0uunits
0u10.0 / 100 units (10%)100u

Protocols

Anti-Aging Skincare Peptide Stack
Beginner
✨Skin & Beauty
Ongoing (8-12 weeks for visible results)

Topical peptide protocol for wrinkle reduction, collagen stimulation, and skin rejuvenation.

Dosage
Matrixyl serum (morning), Argireline serum (evening), GHK-Cu (night)
Frequency
Matrixyl: AM after cleansing. Argireline: PM on expression lines. GHK-Cu: PM after Argireline.
Cycle
Continuous use. Results visible after 8-12 weeks of consistent application.
Stacking Notes
Apply to clean skin. Layer from thinnest to thickest. Use sunscreen daily. Can add oral collagen (10g daily) for enhanced effects.

Warning: Patch test first. Rare sensitivity possible.

View Full Protocol

Stability & Storage

Stable in aqueous solution at pH 5-7. Store cosmetic formulations at room temperature. Lyophilized powder at -20°C. Compatible with most cosmetic ingredients. Penetration enhancers improve topical delivery.

Side Effects & Precautions

Excellent topical safety profile. No systemic absorption at cosmetic concentrations. Rare skin irritation in sensitive individuals. No muscle paralysis risk unlike botulinum toxin.

Research Use Only. This information is for educational and research purposes only. Not intended for medical advice or self-medication.

Regulatory Status

Approved

Approved cosmetic ingredient (INCI: Acetyl Hexapeptide-8). Not a drug — regulated as a cosmetic ingredient. Available in commercial skincare products worldwide.

Research Studies

Argireline Decreases Expression Wrinkle Depth

Blanes-Mira C, Clemente J, Jodas G, et al.

International Journal of Cosmetic Science
2002
View Source

SNAP-25 Fragment Peptide as Anti-Wrinkle Agent

Ruiz MA, Clares B, Morales ME, et al.

Journal of Cosmetic Dermatology
2010
View Source
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