Anti-Aging
Beginner

NAD+ Restoration Protocol

Protocol for restoring cellular NAD+ levels using one of three interchangeable approaches: direct NAD+ administration, NMN supplementation, or Nicotinamide Riboside (NR). Choose ONE approach based on your budget, preferred route, and goals. NAD+, NMN, and NR all raise NAD+ levels — they are alternatives, not a stack. NAD+ levels decline ~50% between ages 40-60, contributing to aging, cognitive decline, and metabolic dysfunction. Restoring NAD+ supports DNA repair, mitochondrial function, sirtuin activation, and cellular energy production.

Duration

Ongoing — NAD+ levels return to baseline within 2-4 weeks after discontinuation. Most users supplement continuously as part of a longevity regimen.

Frequency

Daily for oral supplements (NMN/NR), 1-3x per week for injections (NAD+)

Peptides

3 peptides

Goal

Anti-Aging

Peptides in Protocol

Overview

Protocol for restoring cellular NAD+ levels using one of three interchangeable approaches: direct NAD+ administration, NMN supplementation, or Nicotinamide Riboside (NR). Choose ONE approach based on your budget, preferred route, and goals. NAD+, NMN, and NR all raise NAD+ levels — they are alternatives, not a stack. NAD+ levels decline ~50% between ages 40-60, contributing to aging, cognitive decline, and metabolic dysfunction. Restoring NAD+ supports DNA repair, mitochondrial function, sirtuin activation, and cellular energy production.

Peptides Involved

Compare the peptides included in this protocol side by side.

Typical Dose
NAD+
Approved
Longevity500 mcg 1-4 sessions per week in clinical settings
NMN
Approved
Longevity334.22 Da500 mcg Once daily, preferably in the morning
Nicotinamide Riboside
Approved
Longevity255.25 Da500 mcg Once or twice daily with food

Comparing 3 peptides

Dosage Schedule

Dosage

Option A (NMN oral — most popular): 250-500 mg daily in the morning. Option B (NR oral — best researched): 250-500 mg daily with food. Option C (NAD+ injection — most potent): 50-100 mg subcutaneous 1-2x/week. Option D (NAD+ IV — clinical setting): 250-500 mg infusion 1x/week.

Frequency

Daily for oral supplements (NMN/NR), 1-3x per week for injections (NAD+)

Cycle Information

Ongoing — NAD+ levels return to baseline within 2-4 weeks after discontinuation. Most users supplement continuously as part of a longevity regimen.

No cycling required for oral NMN/NR. For NAD+ injections: some practitioners recommend 4 weeks on, 1 week off. Loading phase option: higher doses for 7-10 days initially, then maintenance.

Stacking Notes

Recommended additions: Resveratrol (500-1000 mg) — activates sirtuins that depend on NAD+. TMG / Trimethylglycine (500-1000 mg) — replenishes methyl donors depleted by NAD+ metabolism. Do NOT combine multiple NAD+ precursors (e.g. NMN + NR) — choose one. Can be combined with other longevity peptides (Epithalon, MOTS-c) in broader anti-aging protocols.

Peptides in this stack::

Warnings & Precautions

Consult a physician before starting, especially with cancer history (NAD+ may theoretically support cancer cell proliferation). Avoid during pregnancy/breastfeeding. NAD+ IV infusion may cause temporary flushing, nausea, or headache — slow infusion rate helps. Start with lower doses to assess individual tolerance.

Research Use Only. This information is for educational and research purposes only. Not intended for medical advice or self-medication.

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