Peptide Stability and Storage: practical guide

Proper storage and handling of peptides is crucial for maintaining their biological activity and research integrity. This guide provides evidence-based recommendations:

Storing lyophilized powder

Lyophilized (Powder) Storage:

• Store at -20°C to -80°C
• Keep in original sealed vial with desiccant
• Stability: typically 2-3 years when properly stored
• Avoid repeated freeze-thaw cycles

Storing reconstituted solutions

Reconstituted Solution Storage:

• Refrigerate at 2-8°C for short-term (up to 2 weeks)
• Freeze at -20°C for longer storage (up to 3 months)
• Use bacteriostatic water for extended stability
• Aliquot into smaller portions to minimize freeze-thaw

Handling best practices

Handling Best Practices:

• Always use sterile techniques
• Protect from light exposure
• Avoid vigorous shaking; gently swirl
• Allow to reach room temperature before reconstitution
• Record reconstitution date and concentration

Summary

Following these guidelines ensures optimal peptide stability and reproducible research results.